A gold color metal and enamel device 1 1/8 inches (2.86 cm) in height overall consisting of a white tapered vertical staff terminating at top in a white fleur-de-lis, entwined below the top by a maroon serpent, all in front of a gold spray of oak on the left and a gold spray of laurel on the right, both enclosing gold rays and all below the upper part of the serpent; arched convexly across the base and looped behind the lower end of the staff a maroon scroll inscribed "TO FIGHT" on the left and "FOR LIFE" on the right in gold letters.
Maroon and white are colors used for the Medical Department. The 325th General Hospital's World War II service in Europe, for which it was awarded the Meritorious Unit Commendation, is symbolized by the laurel, an emblem of merit. The laurel, the fleur-de-lis, and the oak are indicative of the area of the campaign. The acorn is also a symbol of life. The staff entwined with the serpent refers to the Staff of Aesculapius and is historically associated with medical services. The gold rays signify the radiant light of knowledge provided by the unit in its observations and studies of patients for effective healing.
The distinctive unit insignia was originally approved for the 325th General Hospital on 10 March 1970. It was redesignated for the 325th Field Hospital with description and symbolism revised on 6 July 1994. The insignia was redesignated for the 325th Combat Support Hospital, with the symbolism revised, on 13 November 2006.